Research — Molecular Microbiology

M30 – MOLECULAR MICROBIOLOGY

MOLECULAR MICROBIOLOGY RESEARCH OPPORTUNITIES (M30 900)

(M30 900)
Cross-listed with L41 (Bio) 590

Stephen M. Beverley, PhD
9th Floor, McDonnell Pediatric Research Building
314-747-2630
Molecular genetics of protozoan parasites and tropical diseases; biosynthesis of the parasite surface, genomics, virulence and drug action or resistance.

Michael Caparon, PhD
10th Floor, McDonnell Pediatric Research Building
314-362-1485
Molecular genetics and pathogenicity of the streptococci and other pathogenic gram positive bacteria.

Tamara L. Doering, MD, PhD
10th Floor, McDonnell Pediatric Research Building
314-747-5597
The Doering lab studies the opportunistic fungal pathogen, Cryptococcus neoformans,with the dual motivations of elucidating basic biology and identifying potential drug targets. Projects include studies of the synthesis and regulation of the main cryptococcal virulence factor, its polysaccharide capsule, and investigation of host fungal interactions. Current approaches include those of biochemistry, cell and molecular biology, and genetics; studies also include high-throughput analysis of host-pathogen interactions and computational approaches to reconstructing the capsule regulatory network.

Daniel Goldberg, MD, PhD
9th Floor, McDonnell Pediatric Research Building
314-362-1514
Biochemistry of malaria.

Henry Huang, PhD
8th Floor, McDonnell Pediatric Research Building
314-362-2755
RNA virus evolution. Molecular biology of alphaviruses. Alphavirus gene expression vectors. Antiviral drug design.

Scott J. Hultgren, PhD
10th Floor, McDonnell Pediatric Research Building
314-362-6772
Pathogenic mechanisms and disease outcomes in the urinary tract. Work in the Hultgren lab blends multiple scientific disciplines to elucidate bacterial and host mechanisms that determine the onset, course and outcome of interactions between a host mucosal surface and bacterial pathogens. Using genetics, genomics, biochemistry, structural biology, high-resolution imaging, animal models, clinical studies and combinatorial chemistry, we have illuminated new ways in which intracellular lifestyles and community behavior play critical roles in the pathogenesis of urinary tract infection. We have uncovered new principles of adhesive pili biogenesis in Gram Negative bacteria by the chaperone/usher pathway; delineating the fine molecular details of a donor strand complementation and exchange mechanism by which the energy of final subunit folding is used to complete assembly and extrusion of pili across the outer membrane. We revealed how UPEC use type 1 pili to invade and establish biofilm-like intracellular bacterial communities within bladder cells as part of a mechanism that subverts host defenses and how quiescent intracellular reservoirs can seed recurrent infections. We have uncovered complex networks that govern mucosal epithelial response to infection, which we have shown determines disease outcome. Further, we have made seminal contributions to our understanding of the pathogenesis and response to other uropathogens, polymicrobial infections and catheter-associated UTIs and to the mechanisms by which bacteria form a directed amyloid fiber, curli, which is important in biofilm formation. Together, this work is changing the way UTIs are evaluated, re-shaping models of bacterial infections in general and spawning new technologies to design novel vaccines and anti-microbial therapeutics to diagnose, treat and/or prevent UTIs and their sequelae.

Amanda Lewis, PhD
10th Floor, BJC Institute of Health
314-286-0016
Polymicrobial Infection and Women’s Health. Our lab is using biochemical, cellular and animal models to study infectious processes of the female urogenital tract that involve multiple bacterial species. For example, Bacterial vaginosis (BV) is a polymicrobial imbalance of the vaginal flora characterized by reductions in beneficial lactobacilli and an overgrowth of mostly Gram negative bacteria. BV is the most common of all vaginal infections and is associated with increased risks of adverse pregnancy outcomes and greater susceptibility to sexually transmitted infections. We are collaborating with clinical investigators to define molecular and biochemical processes of BV and identify patient groups most at risk for adverse events. Another active area of study in the lab involves polymicrobial urinary tract infection (UTI). We have developed a mouse model of polymicrobia UTI and are currently defining novel processes, bacterial factors and the impact of host that contribute to susceptibility.

Jennifer Lodge, PhD
McDonnell Pediatric Research Building 10210A
314-286-2125
Antifungal therapy and vaccine development against a fungal pathogen: Cryptococcus neoformans is a significant fungal pathogen, particularly in immunocompromised patients, that causes pulmonary infections and meningoencephalitis. It has been estimated that over 1,000,000 new cases of Cryptococcus occur, with over 650,000 deaths per year, and the majority of these cases are in Africa. Our lab focuses on understanding the structure and the synthesis of the fungal cell wall. We are working on it as a target for antifungal therapies and for vaccine development.

David Sibley, PhD
9th Floor, McDonnell Pediatric Research Building
314-362-8873
We study the intracellular survival mechanisms of protozoan parasites, focusing on the model parasite Toxoplasma gondii. Current approaches include high-resolution microscopy, genetic mapping of virulence traits, comparative genomic analyses and development of animal models for studying pathogenesis and resistance.

Christina L. Stallings, PhD
8th Floor, McDonnell Pediatrics Research Building
314-286-0276
Molecular Pathogenesis of Mycobacteria. Our laboratory integrates in vivo disease modeling, molecular biology, and biochemistry to provide answers to the fundamental biological questions regarding molecular pathogenesis and yield therapeutic strategies for treatment of mycobacterial infections.

Niraj H. Tolia, PhD
8th Floor, McDonnell Pediatric Research Building
314-286-0134
Structural and Mechanistic Studies of Malaria Pathogenesis. Our lab is interested in the molecular events that occur during erythrocyte invasion by Plasmodium parasites. We use the tools of structural biology, biochemistry and biophysics to examine proteins and protein complexes associated with these events.

Joseph P. Vogel, PhD
10th Floor, McDonnell Pediatric Research Building
314-747-1029
Legionella pneumophila, the causative agent of Legionnaires’ pneumonia, replicates inside alveolar macrophages by preventing phagosome-lysosome fusion.

David Wang, PhD
8th Floor, McDonnell Pediatric Research Building
314-286-1123
Discovery and characterization of novel viruses. We use functional genomic technologies to identify novel viruses from a variety of clinical samples from diseases of unexplained etiology. We then use epidemiologic and molecular/cellular strategies to define the relevance of newly identified viruses to human disease. A range of new viruses, including polyomaviruses, astroviruses and picronaviruses are under investigation.